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The RNA yield was determined using a NanoDrop 2000 Spectrophotometer (Thermo Scientific, Waltham, USA), and the integrity was evaluated using agarose gel electrophoresis with ethidium bromide stain

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Interestingly, while agonist activation targets lysyl residues in the 2 AR for ubiquitination, carvedilol triggers MARCH2-mediated ubiquitination of non-lysine residues in the 2 AR

doi: 10.3390/ijms221910370 62

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